Collagen membrane versus platelet rich fibrin membrane in bone graft healing: An experimental study in sheep.
Background and objectives: Guided bone regeneration is a term used to define the use of barrier membranes to promote complete osteogenesis. The aim of study was to histologically com-pare the effect of platelet rich fibrin membrane and resorbable Collagen membrane on bone substitute healing.
Methods: in this experimental study three sheep’s which their weighted between 60-70 kg were used. After intramuscular sedation and local anesthesia, eighteen defects were created about 8 mm in diameter and 5 mm in depth in the lower border of mandible. Six defects on each animal which three in right side and three in left side of the mandible. The defects were filled with Biphasic calcium phosphate bone substitute. The first defect was covered by platelet rich fibrin membrane, the second one covered by Collagen membrane, and third one was left uncovered. The animals were sacrificed at 2, 6, and 12 weeks.
Results: The healing of the grafted defects was histologically studied in all groups, and there was non-significant difference between PRF membrane and collagen membrane in healing of bone substitute at 2, 6, and 12 weeks.There was significant deference between PRF membrane and collagen membrane at 6 week regarding scaffold replacement with mature bone.
Conclusion: The use of PRF membrane shows a good outcome as a low cost biological material capable of improving bone healing defects, grafted with bone substitute.
Elgali, I., Omar, O., Dahlin, C., & Thomsen, P. Guided bone regeneration: materials and biolog-ical mechanisms revisited. European journal of oral sciences, 2017. 125(5), 315-337.
Özan, F., Şençimen, M., Gülses, A., & Ayna, M. Guided bone regeneration technique using hyaluronic acid in oral implantology. A Text-book of Advanced Oral and Maxillofacial Sur-gery: Volume 3. 2016, 3343.
Toffler, M. Guided bone regeneration (GBR) using cortical bone pins in combination with leukocyte-and platelet-rich fibrin (L-PRF). Compend Contin Educ Dent, 2014. 35(3): p. 192-8.
Lee, S.-W. and S.-G. Kim. Membranes for the guided bone regeneration. Maxillofacial plastic and reconstructive surgery. 2014. 36(6):239-246.
Wang, H.-L. and W.J. Carroll. Guided bone regeneration using bone grafts and collagen membranes. Quintessence international, 2001. 32(7).
Salamanca, E., Tsai, C. Y., Pan, Y. H., Lin, Y. T., Huang, H. M., Teng, N. C., ... & Chang, W. J. In vitro and in vivo study of a novel porcine collagen membrane for guided bone regenera-tion. Materials, 2016. 9(11), 949.
Varela, H. A., Oliveira, M. N., Pereira, J., Souza, J. C. M., Pinto, N., & Quirynen, M. Chapter 7 - Plate-let-rich fibrin to incorporate bioactive graft mate-rials, in Nanostructured Biomaterials for Cranio-Maxillofacial and Oral Applications, J.C.M. Souza, et al., Editors. 2018, Elsevier. p. 119-142
Castro AB, Meschi N, Temmerman A, Pinto N, Lambrechts P, Teughels W, Quirynen M. Regener-ative potential of leucocyte‐and platelet‐rich fi-brin. Part B: sinus floor elevation, alveolar ridge preservation and implant therapy. A systematic review. Journal of clinical periodontology. 2017; 44(2):225-34.
Borie, E., Oliví, D. G., Orsi, I. A., Garlet, K., Weber, B., Beltrán, V., & Fuentes, R. Platelet-rich fibrin application in dentistry: a literature re-view. International journal of clinical and experi-mental medicine, 2015. 8(5), 7922.
Mazor, Z., Horowitz, R. A., Del Corso, M., Prasad, H. S., Rohrer, M. D., & Dohan Ehrenfest, D. M. Sinus floor augmentation with simultaneous im-plant placement using Choukroun's platelet‐rich fibrin as the sole grafting material: a radiologic and histologic study at 6 months. Journal of perio-dontology, 2009 . 80(12), 2056-2064.
Lucaciu, O., Gheban, D., Soriţau, O., Băciuţ, M., Câmpian, R. S., & Băciuţ, G. Comparative assess-ment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histo-metria și un scor de vindecare histologică. Romanian Review of Laboratory Medicine, 2015. 23(1).
Choukroun, J., Diss, A., Simonpieri, A., Girard, M. O., Schoeffler, C., Dohan, S. L., ... & Dohan, D. M . Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evalua-tions of PRF effects on bone allograft matura-tion in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006. 101(3):299-303.
Olaechea, A., Mendoza-Azpur, G., Valdivia, E., & Rasperini, G . Biodegradation of three different collagen membranes: A histological study. J of Osseointeg. 2016. 8(2):15-9.
Su, C. Y., Kuo, Y. P., Tseng, Y. H., Su, C. H., & Burnouf, T . In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009. 108(1):56-61.
Uchida, S., Sakai, A., Kudo, H., Otomo, H., Wa-tanuki, M., Tanaka, M., ... & Nakamura, T. Vascu-lar endothelial growth factor is expressed along with its receptors during the healing process of bone and bone marrow after drill-hole injury in rats. Bone. 2003. 32(5):491-501.
Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J. A., Spector, J. A., Gittes, G. K., & Longaker, M. T . VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. American Journal of Physiology-Cell Physiology, 2000. 278(4):C853-C860.
Gassling, V., Douglas, T., Warnke, P. H., Açil, Y., Wiltfang, J., & Becker, S. T . Platelet-rich fibrin membranes as scaffolds for periosteal tissue engi-neering. Clin Oral Implants Res, 2010. 21(5):543-9.
Copyright (c) 2020 Mahdi O. Abdullah (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.