Effects of hyaluronic acid on bone graft healing: An experimental study on sheep.

  • Muhmmed Ahmed Mustafa
  • Othman A. Omar College of Dentistry /Hawler Medical University.
Keywords: Hyaluronic acid, Bone graft, Alloplastic material, Guided bone regeneration

Abstract

Background and Objectives: : Alloplastic is one of known materials have been used to treat bony defects in oral and maxillofacial region, but the healing is much more slower than auto graft . So many interpositional graft materials like hyaluronic acid and bone morphogenetic protein have been introduced to such material to induce osteoinductivity of graft material. This study aimed to evaluate the histological effect of Hyaluronic acid on bone graft substitute.
Patients and methods: An experimental study carried out on 3 domestic sheep 2-4 years old. They divided into two groups, first group (study group): bone graft substitute mixed with hyaluronic acid introduced to the created cavities in the basal bone of mandible. Second group (control group): bone graft substitute were introduced into created cavities in the basal bone of mandible and the defects in both groups were covered with collagen membrane. The samples then sacrificed in deferent period interval 2 weeks, 6 weeks and 12 weeks and the sample collected and sent for histological examination.
Results: all animals survived till the end of study. Both groups were characterized by new bone formation in defects however the bone formation in group 1 significantly preceded.
Conclusion: hyaluronic acid induces the onset of Oral and Maxillofacial department.

References

Pagni G, Kaigler D, Rasperini G, Avila-Ortiz G, Bartel R, Giannobile W. Bone repair cells for craniofacial regeneration. Adv Drug Deliv Rev. 2012 Sep;64(12):1310–9.

https://doi.org/10.1016/j.addr.2012.03.005

Davis RE, Telischi FF. Traumatic facial nerve injuries: review of diagnosis and treatment. J Craniomaxillofac Trauma. 1995; 1(3):30–41.

Pape HC, Evans A, Kobbe P. Autologous bone graft: properties and techniques. J Orthop Trauma. 2010 Mar; 24 Suppl 1:S36-40.

https://doi.org/10.1097/BOT.0b013e3181cec4a1

Zouhary KJ. Bone graft harvesting from distant sites: concepts and techniques. Oral Maxillofac Surg Clin North Am. 2010; 22(3):301–16.

De Long WG, Einhorn TA, Koval K, McKee M, Smith W, Sanders R, et al. Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Joint Surg Am. 2007; 89(3):649–58.

https://doi.org/10.2106/JBJS.F.00465

Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002; 84-A(3):454–64.

https://doi.org/10.1111/j.1399-3089.2012.00694.x

Taba M, Jin Q, Sugai JV, Giannobile WV. Current concepts in periodontal bioengineering. Orthod Craniofac Res. 2005; 8(4):292–302.

https://doi.org/10.1111/j.1601-6343.2005.00352.x

Arpağ OF, Damlar I, Altan A, Tatli U, Günay A, Arpağ OF, et al. To what extent does hyaluronic acid affect healing of xenografts? A histomorphometric study in a rabbit model. J Appl Oral Sci. 2018; 26.

https://doi.org/10.1590/1678-7757-2017-00049.

Bertl K, Bruckmann C, Isberg P-E, Klinge B, Gotfredsen K, Stavropoulos A. Hyaluronan in non-surgical and surgical periodontal therapy: A systematic review. J Clin Periodontol. 2015; 42(3):236–46.

https://doi.org/10.1111/jcpe.12371

Zhao N, Wang X, Qin L, Guo Z, Li D. Effect of molecular weight and concentration of hyaluronan on cell proliferation and osteogenic differentiation in vitro. Biochem Biophys Res Commun. 2015. 25;465(3):569–74.

https://doi.org/10.1016/j.bbrc.2015.08.061

Chang E-J, Kim HJ, Ha J, Kim HJ, Ryu J, Park K-H, et al. Hyaluronan inhibits osteoclast differentiation via Toll-like receptor 4. J Cell Sci. 2007; 120(1):166–76.

https://doi.org/10.1242/jcs.03310

Zhu H, Mitsuhashi N, Klein A, Barsky LW, Weinberg K, Barr ML, et al. The role of the hyaluronan receptor CD44 in mesenchymal stem cell migration in the extracellular matrix. Stem Cells. 2006; 24(4):928–35.

https://doi.org/10.1634/stemcells.2005-0186.

Aslan M, Simsek G, Dayi E. The effect of hyaluronic acid-supplemented bone graft in bone healing: experimental study in rabbits. J Biomater Appl. 2006; 20(3):209–20.

https://doi.org/10.1177/0885328206051047

Omar Naser Ihghaf N, Abdel- Monem Tawfik M, Elhawary Y, Ahmed Mansour N. Osteon II versus biogen in healing of jaw bone defects. Egypt Dent J. 2015; 61:4045:4053.

Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res. 2014. 17; 9(1):18.

https://doi.org/10.1186/1749-799X-9-18

Mescher A. Junqueira’s Basic Histology. 12th Revised edition edition. New York: McGraw-Hill Medical; 2009. 467 p.

Fernández RF, Bucchi C, Navarro P, Beltrán V, Borie E. Bone grafts utilized in dentistry: An analysis of patients’ preferences. BMC Med Ethics. 2015. 20; 16.

Published
2021-02-19
How to Cite
Mustafa, M., & Omar, O. (2021). Effects of hyaluronic acid on bone graft healing: An experimental study on sheep. Erbil Dental Journal (EDJ), 3(2), 98-103. https://doi.org/10.15218/edj.2020.14
Section
Original Articles