The use of Platelet Rich Plasmain combination of guided tissue regeneration (chitosan) for bone defects: homogeneity analysis.
Keywords:PRP, Chitosan, scanning electron microscope (SEM), energy dispersive X-ray (EDS), FTIR
Background and objective Guided bone regeneration is an accepted surgical procedure intended to increase the quantity and quality of host bone in localized defects of the alveolar bone. In
this investigation, a novel combination by mixing of Platelet Rich Plasma (PRP) with the chitosan solution has been prepared.
Method In method Platelet-rich plasma was prepared and mixed with chitosan in three different ratios (80:20, 90:10 and 95:5) the homogeneity status of the utilized samples has been
studied using scanning electron microscopy (SEM), energy dispersive X-ray (EDS) analysis and
FT-IR spectroscopy techniques.
Results The result showed The homogeneity status of the utilized samples has been studied
scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis and FT-IR spectroscopy techniques SEM Bigger cracks and obvious gaps can be seen clearly in the 20:80 ratio sample which shows the adhesion failure between PRP and chitosan in this mixture. By energy dispersive X-ray (EDX) It can be seen, that carbon percentages, which belong to chitosan, is a vital
parameter that shows the optimum percentage for the formation of a homogeneous PRPchitosan compound and FTIR show the homogenous mixing without chemical changing of the
mixture and showed the preservation of the chemical properties of each content.
Conclusion The study concluded The homogeneity status of different ratios of novel material
(mixture of PRP / Chitosan) revealed that 95:5 ratio was preferable to test for biocompatibility
and bone regeneration.
Stacie G Boswell . Brian J. Cole, Emily A.
Sundman, Vasili Karas, and Lisa A. Fortier.
"Platelet-rich plasma: a milieu of bioactive factors." Arthroscopy: The journal of arthroscopic
& related surgery 2012, 28 (3) ; 429-439.doi:
1016/j.arthro.2011.10.018. Epub 2012 Jan
Emma Savitri, Sri Rachmania Juliastuti, Anitarakhmi Handaratri, and Achmad Roesyadi.
"Degradation of chitosan by sonication in verylow-concentration acetic acid." Polymer Degradation and Stability. 2014, 110 : 344-352.
Wen Fan , Wei Yan, Zushun Xu, and Hong Ni.
"Formation mechanism of monodisperse, low
molecular weight chitosan nanoparticles by
ionic gelation technique." Colloids and surfaces
B: Biointerfaces. 2012, 90 ; 21-27. doi: 10.1016/
Kweon, D.-K., Song, S.-B., Park, Y.-Y. Preparation
of water-soluble chitosan/heparin complex and
its application as wound healing accelerator.
Biomaterials. 2003. 24, 1595–1601
Marx, R.E., . Platelet-rich plasma (PRP): what is
PRP and what is not PRP? Implant Dent. 2001;
; 225–228. DOI: 10.1097/00008505-
Lee, K.Y., Jeong, L., Kang, Y.O. Electrospinning of
polysaccharides for regenerative medicine. Adv.
Drug Deliv. Rev. 2009; 61, 1020–1032. https://
Kim, S.E., Park, J.H., Cho, Y.W. et al,. Porous chitosan scaffold containing microspheres loaded
with transforming growth factor-beta1: implications for cartilage tissue engineering. J. Control.
Release Off. J. Control. Release Soc. 2003; 91, 365
–374 DOI: 10.1016/s0168-3659(03)00274-8
Shen, E.-C., Chou, T.-C., Gau, C.-H., et al. Releasing
growth factors from activated human platelets
after chitosan stimulation: a possible bio-material
for platelet-rich plasma preparation. Clin. Oral
Implants Res.2006; 17; 572–578. DOI: 10.1111/
El- hefian A. ESAM, Mohamed Mahmoud Nasef.,
Abdul hamid Yahaya. The Preparation and Characterization of Chitosan / Poly (Vinyl Alcohol)
Blended Films. E-Journal of Chemistry. 2010; 7(4),
Oktay, E.O., Demiralp, Bahtiyar, Demiralp, Burak,
Senel et al. Effects of platelet-rich plasma and
chitosan combination on bone regeneration in
experimental rabbit cranial defects. J. Oral Imp l a n t o l . 2 0 1 0 ; 3 6 ; 175–184. h ttp s://
Nimal, T. R., Gaurav Baranwal, M. C. Bavya, et al.
Anti-staphylococcal activity of injectable nano
tigecycline/chitosan-PRP composite hydrogel
using drosophila melanogaster model for infectious wounds." ACS applied materials & interfaces. 2016, 8 (34); 22074-22083. DOI: 10.1021/
Rafigh, Sayyid Mahdi, and Amir Heydarinasab.
"Mesoporous chitosan–SiO2 nanoparticles: synthesis, characterization, and CO2 adsorption capacity." ACS Sustainable Chemistry & Engineering
0 1 7 , 5 ( 1 1 ) ; 1 0 3 7 9 -10386 h tt p s : / /
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