Effect of Aloe vera gel on the parotid glands of streptozotocin-induced diabetic albino rats: histological and immunohistochemical study.
Keywords:Aloe vera, Streptozotocin induced diabetic, Parotid salivary gland, Ki-67, Caspase-3
Background and Objectives: Diabetes mellitus is a disease of international health threat. Among several plants, Aloe vera extract has antidiabetic action thereby validating the rationale of its use in medicine. This study aimed to evaluate the effect of Aloe vera gel on parotid gland of streptozotocin-induced diabetic male albino rats, and also effect of diabetes on the parotid glands.
Methods: Diabetes mellitus was induced in rats by intraperitoneal injection of 60 mg/kg Streptozotocin dissolved in saline solution. A total of 40 adult albino rats were divided equally into 4 groups. The groups consist of normal untreated group, normal treated with Aloe vera gel group (300mg/kg/day administered orally once daily for 21 consecutive days), untreated diabetes mellitus group and diabetes mellitus treated with Aloe vera gel (300mg/kg/day administered orally once daily for 21consecutive days). The parotid salivary glands of all animals were dissected after 2 months and prepared for histological and immunohistochemical examinations. The change in body weight also was measured.
Results: Histopathological examination of the parotid salivary glands in the diabetic group revealed a loss of the standard glandular architecture, including the acini, duct, and connective tissue stroma. Most of these alterations and degenerative changes disappeared or were markedly decreased in the group treated with Aloe vera gel.
Conclusion: Aloe vera gel had a noticeable antidiabetic effect on Streptozotocin-induced diabetic alterations in the parotid salivary glands of rats. Hence, Aloe vera gel may be beneficial as a dietary supplement for reducing diabetes complications.
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